EFFECTS OF OPTIMUM CONDITIONS FOR EXTRACTION OF PHENOL COMPOUNDS FROM Rhodomyrtus Rommentosa LEAVES
DOI:
https://doi.org/10.51453/2354-1431/2022/791Keywords:
sim leaves, Rhodomyrtus tomentosa, phenolic compound, DPPHAbstract
Optimum conditions for extraction of phenolic compounds and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent of sim leaves (Rhodomyrtus tomentosa) harvested in Song Cong, Thai Nguyen province was determined by the conventional liquid-solid method. The phenolic content and antioxidant activity were affected by solvent type and the concentration of solvent, concentration of HCl used, solid/liquid ratio, temperature and extraction time. The selected conditions for the extraction of antioxidant phenol were as follows: methanol concentration, 60%, acidified with 0.5% HCI; solvent/material ratio 1/30; temperature 70°C and extraction time was 60 min. The obtained polyphenol content and DPPH scavenging activity of 162.33 ± 1.23mg equivalent of gallic acid per gram of dried leaves and 1334.84 ± 14.05 µmol of Trolox equivalent per gram of dried leaves, respectively. These high values indicate that sim leaves can be considered a potential nutraceutical source of antioxidant phenolic compounds in the future.
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