Optimization of the production process of liquid fertilizer from beer fermentation residue by using commercial alcalase enzyme

Authors

  • Kim Hoa Dinh Thi Đại học Nông Lâm - Đại học Thái Nguyên
  • Son Luu Hong Đại học Nông Lâm - Đại học Thái Nguyên
  • Luong Ta Thi Trường Đại học Nông Lâm - Đại học Thái Nguyên; Đại học Queensland
  • Chau Le Minh Đại học Nông Lâm - Đại học Thái Nguyên
  • Duong Duong Ngoc Đại học Nông Lâm - Đại học Thái Nguyên
  • Chi Tran Van Đại học Nông Lâm - Đại học Thái Nguyên
  • Lam Vi Dai Đại học Nông Lâm - Đại học Thái Nguyên
  • Tinh Nguyen Thi Đại học Nông Lâm - Đại học Thái Nguyên
  • Duy Nguyen Van Đại học Nông Lâm - Đại học Thái Nguyên
  • Binh Ngo Xuan Đại học Nông Lâm - Đại học Thái Nguyên

DOI:

https://doi.org/10.51453/2354-1431/2020/375

Keywords:

Enzyme Alcalase, fertilizer, yeast residue, optimization, Box-Behnken

Abstract

The aim of the research is to investigate factors that affect the hydrolysis of beer fermentation residues by using commercial alcalase enzyme. These factors are the appropriate mixture ratio between enzyme and Effective Microorganisms (EM), the ratio of supplemental water, the temperature and the time of the incubation process. The single-factor experiments revealed that the amount of soluble protein achived was highest when these factors were EM 2% + E 1.5%, 1/3, 45ºC, 8 hours correspondingly. On the basis of investigating each factor affecting the hydrolysis conditions, that the study showed that the mixture ratio of enzymes and EM, the ratio of supplemental water, the incubation temperature, and the hydrolysis time were strongly influencing factors to the hydrolysis process. By applying Box- Behnken experiment design model, the optimal condition for the hydrolysis process of beer fermentation residues was found, of which the mixture ratio of enzyme and EM was EM 2% + E 1.5%, the ratio of supplemental water was 1/3, the incubation temperature was 45ºC and the incubation time was 8 hours.

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References

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Published

2021-04-29

How to Cite

Định Thị, K. H., Lưu Hồng, S., Tạ Thị, L., Lê Minh, C., Dương Ngọc, D., Trần Văn, C., Vi Đại, L., Nguyễn Thị, T., Nguyễn Văn, D., & Ngô Xuân, B. (2021). Optimization of the production process of liquid fertilizer from beer fermentation residue by using commercial alcalase enzyme. SCIENTIFIC JOURNAL OF TAN TRAO UNIVERSITY, 6(17), 54–60. https://doi.org/10.51453/2354-1431/2020/375

Issue

Section

Natural Science and Technology

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