Optimization of the production process of liquid fertilizer from beer fermentation residue by using commercial alcalase enzyme
DOI:
https://doi.org/10.51453/2354-1431/2020/375Keywords:
Enzyme Alcalase, fertilizer, yeast residue, optimization, Box-BehnkenAbstract
The aim of the research is to investigate factors that affect the hydrolysis of beer fermentation residues by using commercial alcalase enzyme. These factors are the appropriate mixture ratio between enzyme and Effective Microorganisms (EM), the ratio of supplemental water, the temperature and the time of the incubation process. The single-factor experiments revealed that the amount of soluble protein achived was highest when these factors were EM 2% + E 1.5%, 1/3, 45ºC, 8 hours correspondingly. On the basis of investigating each factor affecting the hydrolysis conditions, that the study showed that the mixture ratio of enzymes and EM, the ratio of supplemental water, the incubation temperature, and the hydrolysis time were strongly influencing factors to the hydrolysis process. By applying Box- Behnken experiment design model, the optimal condition for the hydrolysis process of beer fermentation residues was found, of which the mixture ratio of enzyme and EM was EM 2% + E 1.5%, the ratio of supplemental water was 1/3, the incubation temperature was 45ºC and the incubation time was 8 hours.
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