A STUDY ON THE PROCESS OF POLYSACCHARIDE EXTRACTION FROM MYXOPYRUM SMILACIFOLIUM (WALL.) BLUME ROOTS

Authors

  • Luu Hong Son TNU – University of Agriculture and Forestry
  • Nguyễn Thị Tình TNU – University of Agriculture and Forestry
  • Cao Hong Le TNU – University of Agriculture and Forestry
  • Ta Thi Luong The University of Queensland
  • Trinh Thi Chung TNU – University of Agriculture and Forestry
  • Vi Dai Lam TNU – University of Agriculture and Forestry
  • Dinh Thi Kim Hoa TNU – University of Agriculture and Forestry

DOI:

https://doi.org/10.51453/2354-1431/2021/603

Abstract

Myxopyrum smilacifolium (Wall.) has been demonstrated to contain many bioactive compounds, including polysaccharides, saponin, and flavonoids. This study aimed to determine the effects of the single-factor model on the attraction process as a basis for optimal performance. The obtained extract will be evaluated for its antioxidant capacity. The results of the single-factor when extracting the total polysaccharide from Myxopyrum smilacifolium (Wall.) showed the effects of the solvent concentration, solvent/material ratio, extraction time, and temperature on the extraction process with the corresponding results: ethanol 60% (v/v), 20/1 (ml/g), 75 minutes and 70°C respectively. The optimal conditions have been found for the total polysaccharide extraction from Myxopyrum smilacifolium (Wall.): ethanol concentration 66.58%, solvent/material ratio 20.46/1 (ml/g), extraction time 76.77 min, extraction temperature 70°C, respectively. Under the conditions, the total polysaccharide content of the extract reached 3.34937 mg/g of the raw material of Myxopyrum smilacifolium (Wall.) leaves. The extract from Myxopyrum smilacifolium (Wall.) gave an IC50 value of 522.56 ± 13.67 (µg/ml) when evaluating its antioxidant activity.

Downloads

Download data is not yet available.

References

REFERENCES

[1] Box, G.E.P. and Wilson, K.B., (1951). On the experimental attainment of optimum conditions (with discussion). Journal of the Royal Statistical Society Series B. 13 (1): 1-45.

[2] Quang, B.H., Chinh, V.T. (2011). Plant species are used as a medicine of Jasminum in Vietnam. The 4th National Scientific Conference on ecology and biological resources, p.1260.

[3] Chew, K.K., Ng, S.Y., Thoo, Y.Y., Khoo, M. Z., Wan, Aida, W.M., and Ho, C.W. (2011). Effect of ethanol concentration, extraction time and extractiontemperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica extracts. International Food Research Journal, 18 (4): 1427 - 1435.

[4] Damaso Pauline, Igbonekwu-udoji Reagan Jonas, Hien, L.T.T., Thuy, L.T., Le, C.H., Son, L.H., Lam, V.D., Tinh, N.T., Luong, T.T., Hoa, D.T.K. (2020). Research on the extraction process of total polysaccharides from the ( Myxopyrum smilacifolium (Wall.) Blume) and evaluate the antioxidant activity. Scientific Journal – Tan Trao University, 17:36 – 41.

[5] Foster D. S. and Cornella T. S. (1961). Colorimetric Method of Analysis. Nostrand Company Inc New Jersey, 08, pp. 162.

[6] Binh, N.V., Phuong, P.T., Loi, N.T. (2018). Research on some factors affecting the extraction process of total polysaccharide content in Ganoderma lucidum. Journal of Science and Technology - Thai Nguyen University, No. 180 (04): 3 – 8.

[7] Institute of Medicinal Materials (2006). Methods of studying the pharmacological effects of drugs from herbal. Publishing House of Natural Sciences and Technology, Ha Noi

[8] Bac, V.H. (2018). Research on extraction and immune-enhancing effects of polysaccharides from the leaves of Pseuderanthemum palatiferum (nees) radlk. Journal of Biotechnology, 16 (2): 327-335.

[9] Gopalakrishnan S., Rajameena R. (2013), “Wound healing activity of the ethanol extract of the leaves of Myxopyrum serratulum A.W. Hill in rats”, International Journal of Pharmaceutical Sciences and Drug Research, 22(1), pp.143-147.

Downloads

Published

2022-04-12

How to Cite

Lưu Hồng Sơn, L. H. S., Nguyễn Thị, T., Cao Hong, L., Ta Thi, L., Trinh Thi, C., Vi Dai , L., & Dinh Thi Kim , H. (2022). A STUDY ON THE PROCESS OF POLYSACCHARIDE EXTRACTION FROM MYXOPYRUM SMILACIFOLIUM (WALL.) BLUME ROOTS. SCIENTIFIC JOURNAL OF TAN TRAO UNIVERSITY, 7(24). https://doi.org/10.51453/2354-1431/2021/603

Issue

Section

Natural Science and Technology

Most read articles by the same author(s)

1 2 > >>