A STUDY ON THE PROCESS OF POLYSACCHARIDE EXTRACTION FROM MYXOPYRUM SMILACIFOLIUM (WALL.) BLUME ROOTS
DOI:
https://doi.org/10.51453/2354-1431/2021/603Abstract
Myxopyrum smilacifolium (Wall.) has been demonstrated to contain many bioactive compounds, including polysaccharides, saponin, and flavonoids. This study aimed to determine the effects of the single-factor model on the attraction process as a basis for optimal performance. The obtained extract will be evaluated for its antioxidant capacity. The results of the single-factor when extracting the total polysaccharide from Myxopyrum smilacifolium (Wall.) showed the effects of the solvent concentration, solvent/material ratio, extraction time, and temperature on the extraction process with the corresponding results: ethanol 60% (v/v), 20/1 (ml/g), 75 minutes and 70°C respectively. The optimal conditions have been found for the total polysaccharide extraction from Myxopyrum smilacifolium (Wall.): ethanol concentration 66.58%, solvent/material ratio 20.46/1 (ml/g), extraction time 76.77 min, extraction temperature 70°C, respectively. Under the conditions, the total polysaccharide content of the extract reached 3.34937 mg/g of the raw material of Myxopyrum smilacifolium (Wall.) leaves. The extract from Myxopyrum smilacifolium (Wall.) gave an IC50 value of 522.56 ± 13.67 (µg/ml) when evaluating its antioxidant activity.
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